HNRNPA2B1 Antibody, KO Validated

ProSci
Product Code: PSI-14-069
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-14-069-50uL50uL£505.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using HNRNPA2B1 antibody (14-069) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
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Western blot analysis of extracts from normal (control) and HNRNPA2B1 knockout (KO) 293T cells, using HNRNPA2B1 antibody (14-069) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
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Immunohistochemistry of paraffin-embedded mouse brain using HNRNPA2B1 Antibody (14-069) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse kidney using HNRNPA2B1 Antibody (14-069) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat brain using HNRNPA2B1 Antibody (14-069) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat kidney using HNRNPA2B1 Antibody (14-069) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat liver using HNRNPA2B1 Antibody (14-069) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human colon using HNRNPA2B1 Antibody (14-069) at dilution of 1:200 (40x lens).
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Immunofluorescence analysis of HeLa cells using HNRNPA2B1 antibody (14-069). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using HNRNPA2B1 antibody (14-069) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Western blot analysis of extracts from normal (control) and HNRNPA2B1 knockout (KO) 293T cells, using HNRNPA2B1 antibody (14-069) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunohistochemistry of paraffin-embedded mouse brain using HNRNPA2B1 Antibody (14-069) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using HNRNPA2B1 Antibody (14-069) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using HNRNPA2B1 Antibody (14-069) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using HNRNPA2B1 Antibody (14-069) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat liver using HNRNPA2B1 Antibody (14-069) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using HNRNPA2B1 Antibody (14-069) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of HeLa cells using HNRNPA2B1 antibody (14-069). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
HNRNPA2B1, DKFZp779B0244, FLJ22720, HNRNPA2, HNRNPB1, HNRPA2, HNRPA2B1, HNRPB1, RNPA2, SNRPB1, IBMPFD2
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. This gene has been described to generate two alternatively spliced transcript variants which encode different isoforms.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human HNRNPA2B1 (NP_002128.1).
NCBI Gene ID #:
3181
NCBI Official Name:
heterogeneous nuclear ribonucleoprotein A2/B1
NCBI Official Symbol:
HNRNPA2B1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 37kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
P22626
User NOte:
Optimal dilutions for each application to be determined by the researcher.
VALIDATION:
Antibody is Knockout validated.