NEDD8 Antibody

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ProSci
Product Code: PSI-14-075
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-14-075-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using NEDD8 antibody (14-075) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 60s.
2 / 2
Immunohistochemistry of paraffin-embedded human esophagus using NEDD8 antibody (14-075) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of various cell lines, using NEDD8 antibody (14-075) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 60s.
Immunohistochemistry of paraffin-embedded human esophagus using NEDD8 antibody (14-075) at dilution of 1:100 (40x lens).

Documents

Further Information

Additional Names:
NEDD8, NEDD-8, Neddylin, Ubiquitin-like protein Nedd8
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-81 of human NEDD8 (NP_006147.1).
NCBI Gene ID #:
4738
NCBI Official Name:
neural precursor cell expressed, developmentally down-regulated 8
NCBI Official Symbol:
NEDD8
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 12kDa
Purification:
Affinity purification
Research Area:
Cell Cycle
Swissprot #:
Q15843
User NOte:
Optimal dilutions for each application to be determined by the researcher.