ACSL3 Antibody

ProSci
Product Code: PSI-14-096
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-14-096-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 5
Western blot analysis of extracts of various cell lines, using ACSL3 antibody (14-096) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
2 / 5
Immunohistochemistry of paraffin-embedded human mammary cancer using ACSL3 antibody (14-096) at dilution of 1:100 (40x lens).
3 / 5
Immunohistochemistry of paraffin-embedded human stomach using ACSL3 antibody (14-096) at dilution of 1:100 (40x lens).
4 / 5
Immunohistochemistry of paraffin-embedded rat heart using ACSL3 antibody (14-096) at dilution of 1:100 (40x lens).
5 / 5
Immunohistochemistry of paraffin-embedded mouse heart using ACSL3 antibody (14-096) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using ACSL3 antibody (14-096) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human mammary cancer using ACSL3 antibody (14-096) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using ACSL3 antibody (14-096) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using ACSL3 antibody (14-096) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using ACSL3 antibody (14-096) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
ACSL3, ACS3, FACL3, PRO2194
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme is highly expressed in brain, and preferentially utilizes myristate, arachidonate, and eicosapentaenoate as substrates. The amino acid sequence of this isozyme is 92% identical to that of rat homolog. Two transcript variants encoding the same protein have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 42-300 of human ACSL3 (NP_004448.2).
NCBI Gene ID #:
2181
NCBI Official Name:
acyl-CoA synthetase long-chain family member 3
NCBI Official Symbol:
ACSL3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 75kDa
Purification:
Affinity purification
Research Area:
Signal Transduction
Swissprot #:
O95573
User NOte:
Optimal dilutions for each application to be determined by the researcher.