GAS2 Antibody

ProSci
Product Code: PSI-14-097
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-14-097-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 3
Western blot analysis of extracts of various cell lines, using GAS2 antibody (14-097) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
2 / 3
Immunohistochemistry of paraffin-embedded human liver cancer using GAS2 antibody (14-097) at dilution of 1:200 (40x lens).
3 / 3
Immunofluorescence analysis of HeLa cells using GAS2 antibody (14-097). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using GAS2 antibody (14-097) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human liver cancer using GAS2 antibody (14-097) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of HeLa cells using GAS2 antibody (14-097). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
Growth arrest-specific protein 2, GAS-2, GAS2
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
The protein encoded by this gene is a caspase-3 substrate that plays a role in regulating microfilament and cell shape changes during apoptosis. It can also modulate cell susceptibility to p53-dependent apoptosis by inhibiting calpain activity. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human GAS2 (NP_005247.1).
NCBI Gene ID #:
2620
NCBI Official Name:
Growth arrest-specific protein 2
NCBI Official Symbol:
GAS2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 37kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cell Cycle, Signal Transduction
Swissprot #:
O43903
User NOte:
Optimal dilutions for each application to be determined by the researcher.