HES1 Antibody

ProSci
Product Code: PSI-14-116
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-14-116-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using HES1 antibody (14-116) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.

Western blot analysis of extracts of various cell lines, using HES1 antibody (14-116) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.

Further Information

Additional Names:
HES1, HHL, HRY, HES-1, bHLHb39
Application Note:
WB: 1:500 - 1:2000
Background:
This protein belongs to the basic helix-loop-helix family of transcription factors. It is a transcriptional repressor of genes that require a bHLH protein for their transcription. The protein has a particular type of basic domain that contains a helix interrupting protein that binds to the N-box rather than the canonical E-box.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 151-280 of human HES1 (NP_005515.1).
NCBI Gene ID #:
3280
NCBI Official Name:
hes family bHLH transcription factor 1
NCBI Official Symbol:
HES1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 25kDa
Purification:
Affinity purification
Research Area:
Neuroscience, Transcription
Swissprot #:
Q14469
User NOte:
Optimal dilutions for each application to be determined by the researcher.