SERPINI2 Antibody

ProSci
Product Code: PSI-14-121
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-14-121-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 3
Western blot analysis of extracts of various cell lines, using SERPINI2 antibody (14-121) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
2 / 3
Immunofluorescence analysis of rat pancreas using SERPINI2 Polyclonal Antibody (14-121) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
3 / 3
Immunofluorescence analysis of mouse pancreas using SERPINI2 Polyclonal Antibody (14-121) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using SERPINI2 antibody (14-121) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunofluorescence analysis of rat pancreas using SERPINI2 Polyclonal Antibody (14-121) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of mouse pancreas using SERPINI2 Polyclonal Antibody (14-121) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
SERPINI2, MEPI, PI14, PANCPIN, TSA2004, SERPIN12, serine (or cysteine) proteinase inhibitor, clade I (neuroserpin), member 2
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100
Background:
The gene encodes a member of a family of proteins that acts as inhibitors of serine proteases. These proteins function in the regulation of a variety of physiological processes, including coagulation, fibrinolysis, development, malignancy, and inflammation. Expression of the encoded protein may be downregulated during pancreatic carcinogenesis. Alternative splicing results in multiple transcript variants for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 56-405 of human SERPINI2 (NP_006208.1).
NCBI Gene ID #:
5276
NCBI Official Name:
serpin peptidase inhibitor, clade I (pancpin), member 2
NCBI Official Symbol:
SERPINI2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 46kDa
Purification:
Affinity purification
Research Area:
Cell Cycle
Swissprot #:
O75830
User NOte:
Optimal dilutions for each application to be determined by the researcher.