NQO2 Antibody

ProSci
Product Code: PSI-14-126
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-14-126-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using NQO2 antibody (14-126) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded Human liver using NQO2 antibody (14-126) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded Mouse kidney using NQO2 antibody (14-126) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using NQO2 antibody (14-126) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded Human liver using NQO2 antibody (14-126) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Mouse kidney using NQO2 antibody (14-126) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
Ribosyldihydronicotinamide dehydrogenase [quinone], NRH dehydrogenase [quinone] 2, NRH:quinone oxidoreductase 2, Quinone reductase 2, QR2, NQO2, NMOR2
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
This gene encodes a member of the thioredoxin family of enzymes. It is a cytosolic and ubiquitously expressed flavoprotein that catalyzes the two-electron reduction of quinone substrates and uses dihydronicotinamide riboside as a reducing coenzyme. Mutations in this gene have been associated with neurodegenerative diseases and several cancers. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-1-231 of human NQO2 (NP_000895.2).
NCBI Gene ID #:
4835
NCBI Official Name:
Ribosyldihydronicotinamide dehydrogenase [quinone]
NCBI Official Symbol:
NQO2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 26kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Signal Transduction
Swissprot #:
P16083
User NOte:
Optimal dilutions for each application to be determined by the researcher.