FEN1 Antibody

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ProSci
Product Code: PSI-14-128
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-14-128-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Immunohistochemistry of paraffin-embedded human lung cancer using FEN1 antibody (14-128) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse liver using FEN1 antibody (14-128) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of A549 cells using FEN1 antibody (14-128).
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Immunofluorescence analysis of HeLa cells using FEN1 antibody (14-128).
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Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug FEN1 antibody (14-128). Western blot was performed from the immunoprecipitate using FEN1 antibody (14-128) at a dilition of 1:1000.
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Immunohistochemistry of paraffin-embedded human lung cancer using FEN1 antibody (14-128) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using FEN1 antibody (14-128) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using FEN1 antibody (14-128).
Immunofluorescence analysis of HeLa cells using FEN1 antibody (14-128).
Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug FEN1 antibody (14-128). Western blot was performed from the immunoprecipitate using FEN1 antibody (14-128) at a dilition of 1:1000.

Documents

Further Information

Additional Names:
FEN1, FEN-1, MF1, RAD2
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:10 - 1:100

IP: 1:50 - 1:200
Background:
The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 50-380 of human FEN1 (NP_004102.1).
NCBI Gene ID #:
2237
NCBI Official Name:
flap structure-specific endonuclease 1
NCBI Official Symbol:
FEN1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
P39748
User NOte:
Optimal dilutions for each application to be determined by the researcher.