GADD45A Antibody

ProSci
Product Code: PSI-14-137
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-14-137-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using GADD45A antibody (14-137) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
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Immunohistochemistry of paraffin-embedded rat lung using GADD45A antibody (14-137) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human thyroid cancer using GADD45A antibody (14-137) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse liver using GADD45A antibody (14-137) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of U2OS cells using GADD45A antibody (14-137) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using GADD45A antibody (14-137) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunohistochemistry of paraffin-embedded rat lung using GADD45A antibody (14-137) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human thyroid cancer using GADD45A antibody (14-137) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using GADD45A antibody (14-137) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using GADD45A antibody (14-137) at dilution of 1:100. Blue: DAPI for nuclear staining.

Further Information

Additional Names:
Growth arrest and DNA damage-inducible protein GADD45 alpha, DNA damage-inducible transcript 1 protein, DDIT-1, GADD45A, DDIT1, GADD45
Application Note:
WB: 1:500 - 1:2000
Background:
This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The protein encoded by this gene responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. The DNA damage-induced transcription of this gene is mediated by both p53-dependent and -independent mechanisms. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-165 of human GADD45A (NP_001915.1).
NCBI Gene ID #:
1647
NCBI Official Name:
Growth arrest and DNA damage-inducible protein GADD45 alpha
NCBI Official Symbol:
GADD45A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 20kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cell Cycle, Immunology
Swissprot #:
P24522
User NOte:
Optimal dilutions for each application to be determined by the researcher.