ISG15 Antibody

ProSci
Product Code: PSI-14-152
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-14-152-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using ISG15 antibody (14-152) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
2 / 5
Immunohistochemistry of paraffin-embedded human gastric cancer using ISG15 Antibody (14-152) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded rat brain using ISG15 Antibody (14-152) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human breast cancer using ISG15 Antibody (14-152) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using ISG15 Antibody (14-152) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using ISG15 antibody (14-152) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human gastric cancer using ISG15 Antibody (14-152) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using ISG15 Antibody (14-152) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using ISG15 Antibody (14-152) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using ISG15 Antibody (14-152) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
ISG15, HUCRP, IFI15, ISG15 ubiquitin-like modifier, G1P2, IP17, Ubiquitin-like protein ISG15, UCRP
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
The protein encoded by this gene is a ubiquitin-like protein that is conjugated to intracellular target proteins upon activation by interferon-alpha and interferon-beta. Several functions have been ascribed to the encoded protein, including chemotactic activity towards neutrophils, direction of ligated target proteins to intermediate filaments, cell-to-cell signaling, and antiviral activity during viral infections. While conjugates of this protein have been found to be noncovalently attached to intermediate filaments, this protein is sometimes secreted.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-165 of human ISG15 (NP_005092.1).
NCBI Gene ID #:
9636
NCBI Official Name:
ISG15 ubiquitin-like modifier
NCBI Official Symbol:
ISG15
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 14kDa
Purification:
Affinity purification
Research Area:
Cell Cycle
Swissprot #:
P05161
User NOte:
Optimal dilutions for each application to be determined by the researcher.