CD8A Antibody

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ProSci
Product Code: PSI-14-172
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-14-172-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of mouse pancreas, using CD8A antibody (14-172) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded human tonsil using CD8A antibody (14-172) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse spleen using CD8A antibody (14-172) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of HeLa cells using CD8A antibody (14-172) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of NIH/3T3 cells using CD8A antibody (14-172) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of PC12 cells using CD8A antibody (14-172) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of mouse pancreas, using CD8A antibody (14-172) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human tonsil using CD8A antibody (14-172) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using CD8A antibody (14-172) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using CD8A antibody (14-172) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using CD8A antibody (14-172) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC12 cells using CD8A antibody (14-172) at dilution of 1:100. Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2, CD8a, CD8A, MAL
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 22-182 of human CD8A (NP_001759.3).
NCBI Gene ID #:
925
NCBI Official Name:
CD8a molecule
NCBI Official Symbol:
CD8A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 30kDa
Purification:
Affinity purification
Research Area:
Immunology, Stem Cell
Swissprot #:
P01732
User NOte:
Optimal dilutions for each application to be determined by the researcher.