GGA2 Antibody

ProSci
Product Code: PSI-14-174
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-14-174-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using GGA2 antibody (14-174) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
2 / 5
Immunohistochemistry of paraffin-embedded human kidney using GGA2 Antibody (14-174) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded human esophageal cancer using GGA2 Antibody (14-174) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded human stomach using GGA2 Antibody (14-174) at dilution of 1:200 (40x lens).
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Immunofluorescence analysis of MCF-7 cells using GGA2 antibody (14-174). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using GGA2 antibody (14-174) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human kidney using GGA2 Antibody (14-174) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human esophageal cancer using GGA2 Antibody (14-174) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using GGA2 Antibody (14-174) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of MCF-7 cells using GGA2 antibody (14-174). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
GGA2, KIAA1080, VEAR
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes a member of the Golgi-localized, gamma adaptin ear-containing, ARF-binding (GGA) family. This family includes ubiquitous coat proteins that regulate the trafficking of proteins between the trans-Golgi network and the lysosome. These proteins share an amino-terminal VHS domain which mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma-adaptins. This family member may play a significant role in cargo molecules regulation and clathrin-coated vesicle assembly.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human GGA2 (NP_055859.1).
NCBI Gene ID #:
23062
NCBI Official Name:
golgi-associated, gamma adaptin ear containing, ARF binding protein 2
NCBI Official Symbol:
GGA2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 72-80kDa
Purification:
Affinity purification
Research Area:
Signal Transduction
Swissprot #:
Q9UJY4
User NOte:
Optimal dilutions for each application to be determined by the researcher.