LC3B Antibody

ProSci
Product Code: PSI-14-194
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-14-194-50uL50uL£505.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of 293 cells, using LC3B antibody (14-194).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.

Western blot analysis of extracts of 293 cells, using LC3B antibody (14-194).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.

Further Information

Additional Names:
MAP1LC3B Antibody: LC3B, ATG8F, MAP1LC3B-a, MAP1A/1BLC3, MAP1ALC3, Autophagy-related protein LC3 B
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100

IP: 1:20 - 1:50

Flow: 1:20 - 1:50
Background:
The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant protein of human LC3B
NCBI Gene ID #:
81631
NCBI Official Name:
microtubule-associated protein 1 light chain 3 beta
NCBI Official Symbol:
MAP1LC3B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 20kDa
Purification:
Affinity purification
Research Area:
Autophagy, Cancer, Cell Cycle, Neuroscience, Signal Transduction
Swissprot #:
Q9GZQ8
User NOte:
Optimal dilutions for each application to be determined by the researcher.