HGF Antibody

ProSci
Product Code: PSI-14-198
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-14-198-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 4
Western blot analysis of extracts of various cell lines, using HGF antibody (14-198) at 1:400 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 60s.
2 / 4
Immunohistochemistry of paraffin-embedded rat intestine using HGF antibody (14-198) at dilution of 1:100 (40x lens).
3 / 4
Immunohistochemistry of paraffin-embedded human esophagus using HGF antibody (14-198) at dilution of 1:100 (40x lens).
4 / 4
Immunohistochemistry of paraffin-embedded mouse intestine using HGF antibody (14-198) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using HGF antibody (14-198) at 1:400 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 60s.
Immunohistochemistry of paraffin-embedded rat intestine using HGF antibody (14-198) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using HGF antibody (14-198) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse intestine using HGF antibody (14-198) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
HGF, F-TCF, HGFB, HPTA, SF, DFNB39
Application Note:
WB: 1:200 - 1:1000

IHC: 1:50 - 1:100
Background:
This gene encodes a protein that binds to the hepatocyte growth factor receptor to regulate cell growth, cell motility and morphogenesis in numerous cell and tissue types. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate alpha and beta chains, which form the mature heterodimer. This protein is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. This protein also plays a role in angiogenesis, tumorogenesis, and tissue regeneration. Although the encoded protein is a member of the peptidase S1 family of serine proteases, it lacks peptidase activity. Mutations in this gene are associated with nonsyndromic hearing loss.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 30-285 of human HGF (NP_001010933.1).
NCBI Gene ID #:
3082
NCBI Official Name:
hepatocyte growth factor (hepapoietin A; scatter factor)
NCBI Official Symbol:
HGF
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 110kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Growth Factors, Signal Transduction
Swissprot #:
P14210
User NOte:
Optimal dilutions for each application to be determined by the researcher.