FGF10 Antibody

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ProSci
Product Code: PSI-14-224
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-14-224-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of A-549 cells, using FGF10 antibody (14-224) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
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Immunohistochemistry of paraffin-embedded mouse lung using FGF10 antibody (14-224) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse kidney using FGF10 antibody (14-224) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of A-549 cells, using FGF10 antibody (14-224) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded mouse lung using FGF10 antibody (14-224) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using FGF10 antibody (14-224) at dilution of 1:100 (40x lens).

Documents

Further Information

Additional Names:
Fibroblast growth factor 10, FGF-10, Keratinocyte growth factor 2, FGF10
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein exhibits mitogenic activity for keratinizing epidermal cells, but essentially no activity for fibroblasts, which is similar to the biological activity of FGF7. Studies of the mouse homolog of suggested that this gene is required for embryonic epidermal morphogenesis including brain development, lung morphogenesis, and initiation of lim bud formation. This gene is also implicated to be a primary factor in the process of wound healing.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 40-208 of human FGF10 (NP_004456.1).
NCBI Gene ID #:
2255
NCBI Official Name:
Fibroblast growth factor 10
NCBI Official Symbol:
FGF10
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 24.5kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Chemokines & Cytokines, Growth Factors, Immunology, Signal Transduction, Stem Cell
Swissprot #:
O15520
User NOte:
Optimal dilutions for each application to be determined by the researcher.