STAT1 Antibody

ProSci
Product Code: PSI-14-249
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-14-249-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of HeLa cells, using STAT1 antibody (14-249) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded human mammary cancer using STAT1 antibody (14-249) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human gastric cancer using STAT1 antibody (14-249) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of U2OS cells using STAT1 antibody (14-249) at dilution of 1:100. Blue: DAPI for nuclear staining.
5 / 5
Immunoprecipitation analysis of 100ug extracts of HeLa cells using 3ug STAT1 antibody (14-249). Western blot was performed from the immunoprecipitate using STAT1 antibody (14-249) at a dilition of 1:1000.

Western blot analysis of extracts of HeLa cells, using STAT1 antibody (14-249) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human mammary cancer using STAT1 antibody (14-249) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using STAT1 antibody (14-249) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using STAT1 antibody (14-249) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 100ug extracts of HeLa cells using 3ug STAT1 antibody (14-249). Western blot was performed from the immunoprecipitate using STAT1 antibody (14-249) at a dilition of 1:1000.

Further Information

Additional Names:
STAT1 alpha Antibody: CANDF7, ISGF-3, STAT91
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200

IP: 1:20 - 1:50
Background:
The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 650-750 of human STAT1 (NP_009330.1).
NCBI Gene ID #:
6772
NCBI Official Name:
signal transducer and activator of transcription 1, 91kDa
NCBI Official Symbol:
STAT1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 91kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cancer, Cell Cycle, Immunology, Signal Transduction, Transcription
Swissprot #:
P42224
User NOte:
Optimal dilutions for each application to be determined by the researcher.