ATP6AP1 Antibody

ProSci
Product Code: PSI-14-256
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-14-256-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using ATP6AP1 antibody (14-256) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.

Western blot analysis of extracts of various cell lines, using ATP6AP1 antibody (14-256) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.

Further Information

Additional Names:
16A, ATP6IP1, ATP6S1, Ac45, CF2, VATPS1, XAP-3, XAP3, V-type proton ATPase subunit S1, ATPase, H+ transporting, lysosomal (vacuolar proton pump), subunit 1, ATPase, H+ transporting, lysosomal accessory protein 1, ATPase, H+ transporting, lysosomal interacting protein 1, H-ATPase subunit, V-ATPase Ac45 subunit, V-ATPase S1 accessory protein, V-ATPase subunit S1, epididymis secretory sperm binding protein, protein XAP-3, vacuolar proton pump subunit S1
Application Note:
WB: 1:500 - 1:2000
Background:
This gene encodes a component of a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. Vacuolar ATPase (V-ATPase) is comprised of a cytosolic V1 (site of the ATP catalytic site) and a transmembrane V0 domain. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, and receptor-mediated endocytosis. The encoded protein of this gene may assist in the V-ATPase-mediated acidification of neuroendocrine secretory granules. This protein may also play a role in early development.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 90-390 of human ATP6AP1 (NP_001174.2).
NCBI Gene ID #:
537
NCBI Official Name:
ATPase H+ transporting accessory protein 1
NCBI Official Symbol:
ATP6AP1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 50kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Chemokines & Cytokines, Growth Factors, Immunology
Swissprot #:
Q15904
User NOte:
Optimal dilutions for each application to be determined by the researcher.