NDUFA8 Antibody

ProSci
Product Code: PSI-14-265
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-14-265-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using NDUFA8 antibody (14-265) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
2 / 2
Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug NDUFA8 antibody (14-265). Western blot was performed from the immunoprecipitate using NDUFA8 antibody (14-265) at a dilition of 1:1000.

Western blot analysis of extracts of various cell lines, using NDUFA8 antibody (14-265) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug NDUFA8 antibody (14-265). Western blot was performed from the immunoprecipitate using NDUFA8 antibody (14-265) at a dilition of 1:1000.

Further Information

Additional Names:
CI-19KD, CI-PGIV, PGIV, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8, NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 8, 19kDa, NADH-ubiquinone oxidoreductase 19 kDa subunit, NADH:ubiquinone oxidoreductase PGIV subunit, complex I-19kD, complex I-PGIV
Application Note:
WB: 1:500 - 1:2000

IP: 1:50 - 1:100
Background:
The protein encoded by this gene belongs to the complex I 19 kDa subunit family. Mammalian complex I is composed of 45 different subunits. This protein has NADH dehydrogenase activity and oxidoreductase activity. It plays an important role in transfering electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 33-172 of human NDUFA8 (NP_055037.1).
NCBI Gene ID #:
4702
NCBI Official Name:
NADH:ubiquinone oxidoreductase subunit A8
NCBI Official Symbol:
NDUFA8
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 19kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Neuroscience, Signal Transduction
Swissprot #:
P51970
User NOte:
Optimal dilutions for each application to be determined by the researcher.