SHMT2 Antibody

ProSci
Product Code: PSI-14-280
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-14-280-50uL50uL£440.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using SHMT2 antibody (14-280) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.
2 / 5
Immunohistochemistry of paraffin-embedded rat kidney using SHMT2 Antibody (14-280) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human liver cancer using SHMT2 Antibody (14-280) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human lung cancer using SHMT2 Antibody (14-280) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of HeLa cells using SHMT2 antibody (14-280). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using SHMT2 antibody (14-280) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.
Immunohistochemistry of paraffin-embedded rat kidney using SHMT2 Antibody (14-280) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using SHMT2 Antibody (14-280) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using SHMT2 Antibody (14-280) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using SHMT2 antibody (14-280). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
SHMT2, GLYA, SHMT, HEL-S-51e
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes the mitochondrial form of a pyridoxal phosphate-dependent enzyme that catalyzes the reversible reaction of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. The encoded product is primarily responsible for glycine synthesis. The activity of the encoded protein has been suggested to be the primary source of intracellular glycine. The gene which encodes the cytosolic form of this enzyme is located on chromosome 17. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 265-504 of human SHMT2 (NP_005403.2).
NCBI Gene ID #:
6472
NCBI Official Name:
serine hydroxymethyltransferase 2 (mitochondrial)
NCBI Official Symbol:
SHMT2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 52kDa
Purification:
Affinity purification
Research Area:
Cancer, Signal Transduction
Swissprot #:
P34897
User NOte:
Optimal dilutions for each application to be determined by the researcher.