ARHGAP25 Antibody

ProSci
Product Code: PSI-14-326
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-14-326-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 5
Western blot analysis of extracts of various cell lines, using ARHGAP25 antibody (14-326) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
2 / 5
Immunohistochemistry of paraffin-embedded human kidney using ARHGAP25 antibody (14-326) at dilution of 1:200 (40x lens).
3 / 5
Immunohistochemistry of paraffin-embedded Human gastric using ARHGAP25 antibody (14-326) at dilution of 1:100 (40x lens).
4 / 5
Immunofluorescence analysis of Jurkat cells using ARHGAP25 Polyclonal Antibody (14-326) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
5 / 5
Immunofluorescence analysis of L929 cells using ARHGAP25 Polyclonal Antibody (14-326) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using ARHGAP25 antibody (14-326) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human kidney using ARHGAP25 antibody (14-326) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded Human gastric using ARHGAP25 antibody (14-326) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of Jurkat cells using ARHGAP25 Polyclonal Antibody (14-326) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of L929 cells using ARHGAP25 Polyclonal Antibody (14-326) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
ARHGAP25, KAIA0053, HEL-S-308
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-370 of human ARHGAP25 (NP_001159748.1).
NCBI Gene ID #:
9938
NCBI Official Name:
Rho GTPase activating protein 25
NCBI Official Symbol:
ARHGAP25
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 70kDa
Purification:
Affinity purification
Research Area:
Signal Transduction
Swissprot #:
P42331
User NOte:
Optimal dilutions for each application to be determined by the researcher.