CST8 Antibody

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ProSci
Product Code: PSI-14-329
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-14-329-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using CST8 antibody (14-329) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
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Immunofluorescence analysis of HeLa cells using CST8 antibody (14-329). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using CST8 antibody (14-329) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence analysis of HeLa cells using CST8 antibody (14-329). Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
CST8, CST8, cystatin-related epididymal-specific, cystatin-related epididymal spermatogenic protein, cystatin 8 (cystatin-related epididymal specific), cystatin 8, CRES
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200
Background:
The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes a protein similar to type 2 cystatins. The encoded protein exhibits highly tissue-specific expression in the reproductive tract, suggesting implicit roles in reproduction. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 53-142 of human CST8 (NP_005483.1).
NCBI Gene ID #:
10047
NCBI Official Name:
cystatin 8 (cystatin-related epididymal specific)
NCBI Official Symbol:
CST8
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 27kDa
Purification:
Affinity purification
Research Area:
Cell Cycle
Swissprot #:
O60676
User NOte:
Optimal dilutions for each application to be determined by the researcher.