USP4 Antibody

ProSci
Product Code: PSI-14-331
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-14-331-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 1
Western blot analysis of extracts of various cell lines, using USP4 antibody (14-331) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.

Western blot analysis of extracts of various cell lines, using USP4 antibody (14-331) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.

Further Information

Additional Names:
USP4, ubiquitin specific protease 4 (proto-oncogene), UNP, Unph, ubiquitin specific protease, proto-oncogene, UNPH
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
The protein encoded by this gene is a protease that deubiquitinates target proteins such as ADORA2A and TRIM21. The encoded protein shuttles between the nucleus and cytoplasm and is involved in maintaining operational fidelity in the endoplasmic reticulum. Three transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 560-700 of human USP4 (NP_003354.2).
NCBI Gene ID #:
7375
NCBI Official Name:
ubiquitin specific peptidase 4 (proto-oncogene)
NCBI Official Symbol:
USP4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 95kDa
Purification:
Affinity purification
Research Area:
Cell Cycle
Swissprot #:
Q13107
User NOte:
Optimal dilutions for each application to be determined by the researcher.