AMPK alpha 1 Antibody

Contact us
ProSci
Product Code: PSI-14-346
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-14-346-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 2
Western blot analysis of extracts of various cell lines, using PRKAA1 antibody (14-346) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.
2 / 2
Immunofluorescence analysis of HeLa cells using AMPK alpha 1 antibody (14-346). Blue: DAPI for nuclear staining.
❮❯

Western blot analysis of extracts of various cell lines, using PRKAA1 antibody (14-346) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.
Immunofluorescence analysis of HeLa cells using AMPK alpha 1 antibody (14-346). Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
PRKAA1, AMPK, AMPKa1, MGC33776, MGC57364
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200
Background:
The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human AMPK alpha 1 (NP_006242.5).
NCBI Gene ID #:
5562
NCBI Official Name:
protein kinase, AMP-activated, alpha 1 catalytic subunit
NCBI Official Symbol:
PRKAA1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 65kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Autophagy, Cancer, Cell Cycle, Neuroscience, Signal Transduction
Swissprot #:
Q13131
User NOte:
Optimal dilutions for each application to be determined by the researcher.