SDC1 Antibody

ProSci
Product Code: PSI-14-373
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-14-373-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using SDC1 antibody (14-373) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
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Immunohistochemistry of paraffin-embedded human kidney cancer using SDC1 antibody (14-373) at dilution of 1:200 (40x lens).

Western blot analysis of extracts of various cell lines, using SDC1 antibody (14-373) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human kidney cancer using SDC1 antibody (14-373) at dilution of 1:200 (40x lens).

Further Information

Additional Names:
SDC1, syndecan 1, CD138, SDC, SYND1, syndecan, CD138 antigen, heparan sulfate proteoglycan fibroblast growth factor receptor, syndecan proteoglycan 1, syndecan-1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. The syndecans mediate cell binding, cell signaling, and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan-1 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. Altered syndecan-1 expression has been detected in several different tumor types. While several transcript variants may exist for this gene, the full-length natures of only two have been described to date. These two represent the major variants of this gene and encode the same protein.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 25-240 of human SDC1 (NP_002988.3).
NCBI Gene ID #:
6382
NCBI Official Name:
syndecan 1
NCBI Official Symbol:
SDC1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 32kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Immunology, Neuroscience, Signal Transduction, Stem Cell
Swissprot #:
P18827
User NOte:
Optimal dilutions for each application to be determined by the researcher.