UBA1 Antibody

ProSci
Product Code: PSI-14-377
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-14-377-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using UBA1 antibody (14-377) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
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Immunohistochemistry of paraffin-embedded Rat liver using UBA1 antibody (14-377) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded Human colon carcinoma using UBA1 antibody (14-377) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded Mouse brain using UBA1 antibody (14-377) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of HT-29 cells using UBA1 antibody (14-377) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using UBA1 antibody (14-377) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
Immunohistochemistry of paraffin-embedded Rat liver using UBA1 antibody (14-377) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Human colon carcinoma using UBA1 antibody (14-377) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Mouse brain using UBA1 antibody (14-377) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HT-29 cells using UBA1 antibody (14-377) at dilution of 1:100. Blue: DAPI for nuclear staining.

Further Information

Additional Names:
Ubiquitin-like modifier-activating enzyme 1, Protein A1S9, Ubiquitin-activating enzyme E1, UBA1, A1S9T, UBE1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
The protein encoded by this gene catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. This gene complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair. It is part of a gene cluster on chromosome Xp11.23. Alternatively spliced transcript variants that encode the same protein have been described.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 300-600 of human UBA1 (NP_695012.1).
NCBI Gene ID #:
7317
NCBI Official Name:
Ubiquitin-like modifier-activating enzyme 1
NCBI Official Symbol:
UBA1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 121kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Neuroscience
Swissprot #:
P22314
User NOte:
Optimal dilutions for each application to be determined by the researcher.