Iba1 Antibody

ProSci
Product Code: PSI-14-395
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-14-395-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of rat spleen, using Iba1 antibody (14-395) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded rat spinal cord using Iba1 antibody (14-395) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat spleen using Iba1 antibody (14-395) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse spleen using Iba1 antibody (14-395) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of rat spleen, using Iba1 antibody (14-395) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat spinal cord using Iba1 antibody (14-395) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat spleen using Iba1 antibody (14-395) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using Iba1 antibody (14-395) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
AIF1, allograft inflammatory factor 1, IBA1, AIF-1, IRT-1, interferon gamma responsive transcript, ionized calcium-binding adapter molecule, Daintain, G1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100
Background:
This gene encodes a protein that binds actin and calcium. This gene is induced by cytokines and interferon and may promote macrophage activation and growth of vascular smooth muscle cells and T-lymphocytes. Polymorphisms in this gene may be associated with systemic sclerosis. Alternative splicing results in multiple transcript variants, but the full-length and coding nature of some of these variants is not certain.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-147 of human Iba1 (NP_001614.3).
NCBI Gene ID #:
199
NCBI Official Name:
allograft inflammatory factor 1
NCBI Official Symbol:
AIF1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 16kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Immunology, Innate Immunity, Neuroscience
Swissprot #:
P55008
User NOte:
Optimal dilutions for each application to be determined by the researcher.