PRSS1 Antibody

ProSci
Product Code: PSI-14-411
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-14-411-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using PRSS1 antibody (14-411) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
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Immunohistochemistry of paraffin-embedded human lung cancer using PRSS1 antibody (14-411) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human prostate using PRSS1 antibody (14-411) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse lung using PRSS1 antibody (14-411) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using PRSS1 antibody (14-411) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human lung cancer using PRSS1 antibody (14-411) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate using PRSS1 antibody (14-411) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using PRSS1 antibody (14-411) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
Trypsin-1, Beta-trypsin, Cationic trypsinogen, Serine protease 1, Trypsin I, Alpha-trypsin chain 1, Alpha-trypsin chain 2, PRSS1, TRP1, TRY1, TRYP1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:100 - 1:200
Background:
This gene encodes a trypsinogen, which is a member of the trypsin family of serine proteases. This enzyme is secreted by the pancreas and cleaved to its active form in the small intestine. It is active on peptide linkages involving the carboxyl group of lysine or arginine. Mutations in this gene are associated with hereditary pancreatitis. This gene and several other trypsinogen genes are localized to the T cell receptor beta locus on chromosome 7.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 24-247 of human PRSS1 (NP_002760.1).
NCBI Gene ID #:
5644
NCBI Official Name:
Trypsin-1
NCBI Official Symbol:
PRSS1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 35kDa
Purification:
Affinity purification
Research Area:
Cell Cycle
Swissprot #:
P07477
User NOte:
Optimal dilutions for each application to be determined by the researcher.