HNRNPL Antibody

ProSci
Product Code: PSI-14-428
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-14-428-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using HNRNPL antibody (14-428) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded rat lung using HNRNPL antibody (14-428) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat spleen using HNRNPL antibody (14-428) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human thyroid cancer using HNRNPL antibody (14-428) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human esophageal using HNRNPL antibody (14-428) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human placenta using HNRNPL antibody (14-428) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using HNRNPL antibody (14-428) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of 293T cells using HNRNPL antibody (14-428) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using HNRNPL antibody (14-428) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat lung using HNRNPL antibody (14-428) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat spleen using HNRNPL antibody (14-428) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human thyroid cancer using HNRNPL antibody (14-428) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophageal using HNRNPL antibody (14-428) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human placenta using HNRNPL antibody (14-428) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using HNRNPL antibody (14-428) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of 293T cells using HNRNPL antibody (14-428) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
HNRPL, HNRPL, hnRNP-L, P/OKcl.14
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
Heterogeneous nuclear RNAs (hnRNAs) which include mRNA precursors and mature mRNAs are associated with specific proteins to form heterogenous ribonucleoprotein (hnRNP) complexes. Heterogeneous nuclear ribonucleoprotein L is among the proteins that are stably associated with hnRNP complexes and along with other hnRNP proteins is likely to play a major role in the formation, packaging, processing, and function of mRNA. Heterogeneous nuclear ribonucleoprotein L is present in the nucleoplasm as part of the HNRP complex. HNRP proteins have also been identified outside of the nucleoplasm. Exchange of hnRNP for mRNA-binding proteins accompanies transport of mRNA from the nucleus to the cytoplasm. Since HNRP proteins have been shown to shuttle between the nucleus and the cytoplasm, it is possible that they also have cytoplasmic functions. Two transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 282-589 of human HNRNPL (NP_001524.2).
NCBI Gene ID #:
3191
NCBI Official Name:
heterogeneous nuclear ribonucleoprotein L
NCBI Official Symbol:
HNRNPL
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 64kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
P14866
User NOte:
Optimal dilutions for each application to be determined by the researcher.