HSP90AA1 Antibody

ProSci
Product Code: PSI-14-429
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-14-429-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Immunofluorescence analysis of C6 cells using HSP90AA1 antibody (14-429) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of NIH-3T3 cells using HSP90AA1 antibody (14-429) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunohistochemistry of paraffin-embedded mouse brain using HSP90AA1 antibody (14-429) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse testis using HSP90AA1 antibody (14-429) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded rat brain using HSP90AA1 antibody (14-429) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded rat testis using HSP90AA1 antibody (14-429) at dilution of 1:200 (40x lens).
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Western blot analysis of extracts of various cell lines, using HSP90AA1 antibody (14-429) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.

Immunofluorescence analysis of C6 cells using HSP90AA1 antibody (14-429) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using HSP90AA1 antibody (14-429) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunohistochemistry of paraffin-embedded mouse brain using HSP90AA1 antibody (14-429) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using HSP90AA1 antibody (14-429) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using HSP90AA1 antibody (14-429) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat testis using HSP90AA1 antibody (14-429) at dilution of 1:200 (40x lens).
Western blot analysis of extracts of various cell lines, using HSP90AA1 antibody (14-429) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.

Further Information

Additional Names:
HSP90, HSP90A, HSPC1, HSPCA, Heat shock protein HSP 90-alpha, Heat shock 86 kDa, HSP 86, EL52, HSPN, LAP2, HSP86, Hsp89, Hsp90, HSP89A, HSP90N, HSPCAL1, HSPCAL4
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
The protein encoded by this gene is an inducible molecular chaperone that functions as a homodimer. The encoded protein aids in the proper folding of specific target proteins by use of an ATPase activity that is modulated by co-chaperones. Two transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide of human HSP90AA1
NCBI Gene ID #:
3320
NCBI Official Name:
heat shock protein 90kDa alpha (cytosolic), class A member 1
NCBI Official Symbol:
HSP90AA1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 101kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cancer, Cell Cycle, Immunology, Signal Transduction
Swissprot #:
P07900
User NOte:
Optimal dilutions for each application to be determined by the researcher.