NTF3 Antibody

ProSci
Product Code: PSI-14-446
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-14-446-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using NTF3 antibody (14-446) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 3
Immunohistochemistry of paraffin-embedded mouse brain using NTF3 antibody (14-446) (40x lens).
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Immunohistochemistry of paraffin-embedded human liver damage using NTF3 antibody (14-446) (40x lens).

Western blot analysis of extracts of various cell lines, using NTF3 antibody (14-446) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded mouse brain using NTF3 antibody (14-446) (40x lens).
Immunohistochemistry of paraffin-embedded human liver damage using NTF3 antibody (14-446) (40x lens).

Further Information

Additional Names:
NTF3, neurotrophin 3, HDNF, MGC129711, NGF-2, NGF2, NT3, NT-3
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
The protein encoded by this gene is a member of the neurotrophin family, that controls survival and differentiation of mammalian neurons. This protein is closely related to both nerve growth factor and brain-derived neurotrophic factor. It may be involved in the maintenance of the adult nervous system, and may affect development of neurons in the embryo when it is expressed in human placenta. NTF3-deficient mice generated by gene targeting display severe movement defects of the limbs. The mature peptide of this protein is identical in all mammals examined including human, pig, rat and mouse.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 19-257 of human NTF3 (NP_002518.1).
NCBI Gene ID #:
4908
NCBI Official Name:
neurotrophin 3
NCBI Official Symbol:
NTF3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 36kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cell Cycle, Growth Factors, Neuroscience
Swissprot #:
P20783
User NOte:
Optimal dilutions for each application to be determined by the researcher.