SERPINA1 Antibody

ProSci
Product Code: PSI-14-451
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-14-451-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using SERPINA1 antibody (14-451) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
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Immunohistochemistry of paraffin-embedded human liver cancer using SERPINA1 antibody (14-451) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human esophagus using SERPINA1 antibody (14-451) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of PC-12 cells using SERPINA1 antibody (14-451) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using SERPINA1 antibody (14-451) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human liver cancer using SERPINA1 antibody (14-451) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using SERPINA1 antibody (14-451) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of PC-12 cells using SERPINA1 antibody (14-451) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
SERPINA1, PI, A1A, AAT, PI1, A1AT, PRO2275, alpha1AT
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
The protein encoded by this gene is secreted and is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. Defects in this gene can cause emphysema or liver disease. Several transcript variants encoding the same protein have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 25-315 of human SERPINA1 (NP_000286.3).
NCBI Gene ID #:
5265
NCBI Official Name:
serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1
NCBI Official Symbol:
SERPINA1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 45kDa,50kDa
Purification:
Affinity purification
Research Area:
Cancer
Swissprot #:
P01009
User NOte:
Optimal dilutions for each application to be determined by the researcher.