Akt2 Antibody

ProSci
Product Code: PSI-14-464
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-14-464-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of 293 cells, using Akt2 antibody (14-464).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
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Immunohistochemistry of paraffin-embedded human breast using AKT2 antibody (14-464) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded rat brain using AKT2 antibody (14-464) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse testis using AKT2 antibody (14-464) at dilution of 1:200 (40x lens).
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Immunofluorescence analysis of C6 cells using AKT2 antibody (14-464) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of U2OS cells using AKT2 antibody (14-464) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of 293 cells, using Akt2 antibody (14-464).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human breast using AKT2 antibody (14-464) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using AKT2 antibody (14-464) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using AKT2 antibody (14-464) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of C6 cells using AKT2 antibody (14-464) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using AKT2 antibody (14-464) at dilution of 1:100. Blue: DAPI for nuclear staining.

Further Information

Additional Names:
rac protein kinase beta, Murine thymoma viral (v-akt) homolog-2, RAC-BETA, PRKBB, PKBBETA, HGNC:392, v-akt murine thymoma viral oncogene homolog 2, AKT2, PKBB, HIHGHH
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. The gene was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant protein of human Akt2
NCBI Gene ID #:
208
NCBI Official Name:
v-akt murine thymoma viral oncogene homolog 2
NCBI Official Symbol:
AKT2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 52kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cancer, Cell Cycle, Immunology, Neuroscience, Signal Transduction
Swissprot #:
P31751
User NOte:
Optimal dilutions for each application to be determined by the researcher.