TOP1 Antibody

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ProSci
Product Code: PSI-14-483
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-14-483-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using TOP1 antibody (14-483) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded mouse liver using TOP1 antibody (14-483) at dilution of 1:100 (40x lens).
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Immunoprecipitation analysis of 200ug extracts of Jurkat cells, using 3 ug TOP1 antibody (14-483). Western blot was performed from the immunoprecipitate using TOP1 antibody (14-483) at a dilition of 1:1000.
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Western blot analysis of extracts of various cell lines, using TOP1 antibody (14-483) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded mouse liver using TOP1 antibody (14-483) at dilution of 1:100 (40x lens).
Immunoprecipitation analysis of 200ug extracts of Jurkat cells, using 3 ug TOP1 antibody (14-483). Western blot was performed from the immunoprecipitate using TOP1 antibody (14-483) at a dilition of 1:1000.

Documents

Further Information

Additional Names:
TOPI, DNA topoisomerase 1, Scl-70 antigen, topoisomerase (DNA) I, type I DNA topoisomerase
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IP: 1:20 - 1:50
Background:
This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human TOP1 (NP_003277.1).
NCBI Gene ID #:
7150
NCBI Official Name:
DNA topoisomerase I
NCBI Official Symbol:
TOP1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 120kDa
Purification:
Affinity purification
Research Area:
Cancer
Swissprot #:
P11387
User NOte:
Optimal dilutions for each application to be determined by the researcher.