ABCF2 Antibody

ProSci
Product Code: PSI-14-504
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-14-504-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using ABCF2 antibody (14-504) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
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Immunohistochemistry of paraffin-embedded rat brain using ABCF2 antibody (14-504) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using ABCF2 antibody (14-504) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of U2OS cells using ABCF2 antibody (14-504) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using ABCF2 antibody (14-504) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
Immunohistochemistry of paraffin-embedded rat brain using ABCF2 antibody (14-504) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using ABCF2 antibody (14-504) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using ABCF2 antibody (14-504) at dilution of 1:100. Blue: DAPI for nuclear staining.

Further Information

Additional Names:
ABC28, EST13309HUSSY-18, HUSSY18, ATP-binding cassette sub-family F member 2, ABC-type transport protein, ATP-binding cassette, sub-family F (GCN20), member 2, iron-inhibited ABC transporter 2
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes a member of the ATP-binding cassette (ABC) transporter superfamily. ATP-binding cassette proteins transport various molecules across extra- and intracellular membranes. Alterations in this gene may be involved in cancer progression. Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 3 and 7.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human ABCF2 (NP_005683.2).
NCBI Gene ID #:
10061
NCBI Official Name:
ATP binding cassette subfamily F member 2
NCBI Official Symbol:
ABCF2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 71kDa
Purification:
Affinity purification
Research Area:
Signal Transduction
Swissprot #:
Q9UG63
User NOte:
Optimal dilutions for each application to be determined by the researcher.