TOPBP1 Antibody

ProSci
Product Code: PSI-14-518
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-14-518-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using TOPBP1 antibody (14-518) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 30s.
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Immunohistochemistry of paraffin-embedded mouse lung using TOPBP1 antibody (14-518) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat lung using TOPBP1 antibody (14-518) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using TOPBP1 antibody (14-518) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded mouse lung using TOPBP1 antibody (14-518) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat lung using TOPBP1 antibody (14-518) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
Dpb11, TOP2BP1, DNA topoisomerase 2-binding protein 1, DNA topoisomerase II-beta-binding protein 1, topoisomerase (DNA) II binding protein 1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
This gene encodes a binding protein which interacts with the C-terminal region of topoisomerase II beta. This interaction suggests a supportive role for this protein in the catalytic reactions of topoisomerase II beta through transient breakages of DNA strands.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human TOPBP1 (NP_008958.2).
NCBI Gene ID #:
11073
NCBI Official Name:
DNA topoisomerase II binding protein 1
NCBI Official Symbol:
TOPBP1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 171kDa
Purification:
Affinity purification
Research Area:
Cancer
Swissprot #:
Q92547
User NOte:
Optimal dilutions for each application to be determined by the researcher.