ARFGAP1 Antibody

Contact us
ProSci
Product Code: PSI-14-531
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-14-531-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 4
Western blot analysis of extracts of various cell lines, using ARFGAP1 antibody (14-531) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 3s.
2 / 4
Immunohistochemistry of paraffin-embedded rat brain using ARFGAP1 antibody (14-531) at dilution of 1:100 (40x lens).
3 / 4
Immunohistochemistry of paraffin-embedded human gastric cancer using ARFGAP1 antibody (14-531) at dilution of 1:100 (40x lens).
4 / 4
Immunofluorescence analysis of A549 cells using ARFGAP1 antibody (14-531). Blue: DAPI for nuclear staining.
❮❯

Western blot analysis of extracts of various cell lines, using ARFGAP1 antibody (14-531) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 3s.
Immunohistochemistry of paraffin-embedded rat brain using ARFGAP1 antibody (14-531) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using ARFGAP1 antibody (14-531) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using ARFGAP1 antibody (14-531). Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
ADP-ribosylation factor GTPase-activating protein 1, ARF GAP 1, ADP-ribosylation factor 1 GTPase-activating protein, ARF1 GAP, ARF1-directed GTPase-activating protein, ARFGAP1, ARF1GAP
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
The protein encoded by this gene is a GTPase-activating protein, which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1. The encoded protein promotes hydrolysis of ADP-ribosylation factor 1-bound GTP and is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is required for the fusion of these vesicles with target compartments. The activity of this protein is stimulated by phosphoinosides and inhibited by phosphatidylcholine. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human ARFGAP1 (NP_001268411.1).
NCBI Gene ID #:
55738
NCBI Official Name:
ADP-ribosylation factor GTPase-activating protein 1
NCBI Official Symbol:
ARFGAP1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 42-45kDa
Purification:
Affinity purification
Research Area:
Signal Transduction
Swissprot #:
Q8N6T3
User NOte:
Optimal dilutions for each application to be determined by the researcher.