EIF2AK4 Antibody

ProSci
Product Code: PSI-14-545
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-14-545-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of HepG2 cells, using EIF2AK4 Antibody (14-545) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
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Immunohistochemistry of paraffin-embedded rat testis using EIF2AK4 antibody (14-545) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human breast cancer using EIF2AK4 antibody (14-545) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse testis using EIF2AK4 antibody (14-545) at dilution of 1:100 (20x lens).
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Immunohistochemistry of paraffin-embedded mouse spinal cord using EIF2AK4 antibody (14-545) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using EIF2AK4 antibody (14-545) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of A549 cells using EIF2AK4 antibody (14-545). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of HepG2 cells, using EIF2AK4 Antibody (14-545) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat testis using EIF2AK4 antibody (14-545) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using EIF2AK4 antibody (14-545) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using EIF2AK4 antibody (14-545) at dilution of 1:100 (20x lens).
Immunohistochemistry of paraffin-embedded mouse spinal cord using EIF2AK4 antibody (14-545) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using EIF2AK4 antibody (14-545) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using EIF2AK4 antibody (14-545). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
EIF2AK4, GCN2, GCN2 eIF2alpha kinase, GCN2-like protein, Mgcn2, KIAA1338
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes a member of a family of kinases that phosphorylate the alpha subunit of eukaryotic translation initiation factor-2 (EIF2), resulting in the downregulaton of protein synthesis. The encoded protein responds to amino acid deprivation by binding uncharged transfer RNAs. It may also be activated by glucose deprivation and viral infection. Mutations in this gene have been found in individuals suffering from autosomal recessive pulmonary venoocclusive-disease-2.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human EIF2AK4 (NP_001013725.2).
NCBI Gene ID #:
440275
NCBI Official Name:
eukaryotic translation initiation factor 2 alpha kinase 4
NCBI Official Symbol:
EIF2AK4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 187kDa
Purification:
Affinity purification
Research Area:
Signal Transduction
Swissprot #:
Q9P2K8
User NOte:
Optimal dilutions for each application to be determined by the researcher.