AREG Antibody

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ProSci
Product Code: PSI-14-577
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-14-577-50uL50uL£433.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using AREG antibody (14-577) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded human liver using AREG antibody (14-577) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human tonsil using AREG antibody (14-577) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of various cell lines, using AREG antibody (14-577) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human liver using AREG antibody (14-577) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human tonsil using AREG antibody (14-577) at dilution of 1:100 (40x lens).

Documents

Further Information

Additional Names:
Amphiregulin, AR, Colorectum cell-derived growth factor, CRDGF, AREG, SDGF
Application Note:
WB: 1:1000 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
The protein encoded by this gene is a member of the epidermal growth factor family. It is an autocrine growth factor as well as a mitogen for astrocytes, Schwann cells and fibroblasts. It is related to epidermal growth factor (EGF) and transforming growth factor alpha (TGF-alpha). The protein interacts with the EGF/TGF-alpha receptor to promote the growth of normal epithelial cells, and it inhibits the growth of certain aggressive carcinoma cell lines. It also functions in mammary gland, oocyte and bone tissue development. This gene is associated with a psoriasis-like skin phenotype, and is also associated with other pathological disorders, including various types of cancers and inflammatory conditions.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 20-252 of human AREG (NP_001648.1).
NCBI Gene ID #:
374
NCBI Official Name:
Amphiregulin
NCBI Official Symbol:
AREG
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 44kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Chemokines & Cytokines, Growth Factors, Immunology, Signal Transduction
Swissprot #:
P15514
User NOte:
Optimal dilutions for each application to be determined by the researcher.