ACSL5 Antibody

Contact us
ProSci
Product Code: PSI-14-586
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-14-586-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 2
Western blot analysis of extracts of various cell lines, using ACSL5 antibody (14-586) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
2 / 2
Immunohistochemistry of paraffin-embedded rat kidney using ACSL5 antibody (14-586) at dilution of 1:100 (40x lens).
❮❯

Western blot analysis of extracts of various cell lines, using ACSL5 antibody (14-586) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunohistochemistry of paraffin-embedded rat kidney using ACSL5 antibody (14-586) at dilution of 1:100 (40x lens).

Documents

Further Information

Additional Names:
ACSL5, acyl-CoA synthetase long-chain family member 5, HGNC:16526, ACS2, ACS5, FACL5, fatty-acid-Coenzyme A ligase, long-chain 5, long-chain acyl-CoA synthetase 5, long-chain fatty acid coenzyme A ligase 5, UNQ633/PRO1250
Application Note:
WB: 1:500 - 1:2000

IHC: 1:20 - 1:100
Background:
The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme is highly expressed in uterus and spleen, and in trace amounts in normal brain, but has markedly increased levels in malignant gliomas. This gene functions in mediating fatty acid-induced glioma cell growth. Three transcript variants encoding two different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 500-739 of human ACSL5 (NP_057318.2).
NCBI Gene ID #:
51703
NCBI Official Name:
acyl-CoA synthetase long-chain family member 5
NCBI Official Symbol:
ACSL5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 70-76kDa
Purification:
Affinity purification
Research Area:
Cancer, Signal Transduction
Swissprot #:
Q9ULC5
User NOte:
Optimal dilutions for each application to be determined by the researcher.