AMPKa1/AMPKa2 Antibody

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ProSci
Product Code: PSI-14-595
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-14-595-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of A-549 cells, using PRKAA1/PRKAA2 antibody (14-595).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
2 / 2
Immunofluorescence analysis of HeLa cells using AMPKa1/AMPKa2 Polyclonal Antibody (14-595) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of A-549 cells, using PRKAA1/PRKAA2 antibody (14-595).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence analysis of HeLa cells using AMPKa1/AMPKa2 Polyclonal Antibody (14-595) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
AMPK, AMPKa1, FLK1, VEGFR2, AAPK1, AMPK alpha-1 chain, AMPK-alpha1, HMG-CoA redustase kinase, PRKAA1
Application Note:
WB: 1:500 - 1:1000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant protein of human PRKAA1/PRKAA2
NCBI Gene ID #:
5562/5563
NCBI Official Name:
protein kinase, AMP-activated, alpha 1 catalytic subunit
NCBI Official Symbol:
PRKAA1/PRKAA2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 64kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q13131/P54646
User NOte:
Optimal dilutions for each application to be determined by the researcher.