PARD6A Antibody

ProSci
Product Code: PSI-14-614
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-14-614-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of rat skeletal muscle, using PARD6A antibody (14-614) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 15s.

Western blot analysis of extracts of rat skeletal muscle, using PARD6A antibody (14-614) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 15s.

Further Information

Additional Names:
PARD6A, PAR-6, TAX40, PAR-6A, TIP-40, PAR6alpha, par-6 partitioning defective 6 homolog alpha (C.elegans), Tax interaction protein 40, par-6 (partitioning defective 6, C.elegans) homolog alpha, PAR6A
Application Note:
WB: 1:500 - 1:2000
Background:
This gene is a member of the PAR6 family and encodes a protein with a PSD95/Discs-large/ZO1 (PDZ) domain and a semi-Cdc42/Rac interactive binding (CRIB) domain. This cell membrane protein is involved in asymmetrical cell division and cell polarization processes as a member of a multi-protein complex. The protein also has a role in the epithelial-to-mesenchymal transition (EMT) that characterizes the invasive phenotype associated with metastatic carcinomas. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 256-345 of human PARD6A (NP_001032358.1).
NCBI Gene ID #:
50855
NCBI Official Name:
par-6 family cell polarity regulator alpha
NCBI Official Symbol:
PARD6A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 37kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Signal Transduction, Stem Cell
Swissprot #:
Q9NPB6
User NOte:
Optimal dilutions for each application to be determined by the researcher.