NAT2 Antibody

ProSci
Product Code: PSI-14-619
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-14-619-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using NAT2 antibody (14-619) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 30s.
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Immunohistochemistry of paraffin-embedded rat lung using NAT2 antibody (14-619) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat heart using NAT2 antibody (14-619) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human breast using NAT2 antibody (14-619) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of 293T cells using NAT2 antibody (14-619) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using NAT2 antibody (14-619) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat lung using NAT2 antibody (14-619) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using NAT2 antibody (14-619) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast using NAT2 antibody (14-619) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of 293T cells using NAT2 antibody (14-619) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
NAT2, AAC2, PNAT, NAT-2
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes an enzyme that functions to both activate and deactivate arylamine and hydrazine drugs and carcinogens. Polymorphisms in this gene are responsible for the N-acetylation polymorphism in which human populations segregate into rapid, intermediate, and slow acetylator phenotypes. Polymorphisms in this gene are also associated with higher incidences of cancer and drug toxicity. A second arylamine N-acetyltransferase gene (NAT1) is located near this gene (NAT2).
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 171-290 of human NAT2 (NP_000006.2).
NCBI Gene ID #:
10
NCBI Official Name:
N-acetyltransferase 2 (arylamine N-acetyltransferase)
NCBI Official Symbol:
NAT2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 36kDa
Purification:
Affinity purification
Research Area:
Cancer, Signal Transduction
Swissprot #:
P11245
User NOte:
Optimal dilutions for each application to be determined by the researcher.