PDIA2 Antibody

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ProSci
Product Code: PSI-14-628
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-14-628-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using PDIA2 antibody (14-628) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunofluorescence analysis of NIH/3T3 cells using PDIA2 antibody (14-628) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using PDIA2 antibody (14-628) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunofluorescence analysis of NIH/3T3 cells using PDIA2 antibody (14-628) at dilution of 1:100. Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
protein disulfide-isomerase A2, protein disulfide isomerase-associated 2, protein disulfide isomerase, pancreatic, protein disulfide isomerase A2, pancreatic protein disulfide isomerase, pancreas-specific protein disulfide isomerase, Rho GDP dissociation inhibitor gamma, OTTHUMP00000197166, OTTHUMP00000067350, PDIR, PDIP, PDI, PDA2, LA16c-314G4.2, protein disulfide isomerase family A, member 2, PDIA2
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200
Background:
This gene encodes a member of the disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins that catalyze protein folding and thiol-disulfide interchange reactions. The encoded protein has an N-terminal ER-signal sequence, two catalytically active thioredoxin (TRX) domains, two TRX-like domains and a C-terminal ER-retention sequence. The protein plays a role in the folding of nascent proteins in the endoplasmic reticulum by forming disulfide bonds through its thiol isomerase, oxidase, and reductase activity.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 326-525 of human PDIA2 (NP_006840.2).
NCBI Gene ID #:
64714
NCBI Official Name:
protein disulfide isomerase family A, member 2
NCBI Official Symbol:
PDIA2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 58kDa
Purification:
Affinity purification
Research Area:
Cancer
Swissprot #:
Q13087
User NOte:
Optimal dilutions for each application to be determined by the researcher.