RAP1B Antibody

ProSci
Product Code: PSI-14-702
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-14-702-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using RAP1B antibody (14-702) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 30s.

Western blot analysis of extracts of various cell lines, using RAP1B antibody (14-702) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 30s.

Further Information

Additional Names:
Ras-related protein Rap-1b, GTP-binding protein smg p21B, RAP1B
Application Note:
WB: 1:500 - 1:2000

IHC: 1:100 - 1:200
Background:
This gene encodes a member of the RAS-like small GTP-binding protein superfamily. Members of this family regulate multiple cellular processes including cell adhesion and growth and differentiation. This protein localizes to cellular membranes and has been shown to regulate integrin-mediated cell signaling. This protein also plays a role in regulating outside-in signaling in platelets. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 3, 5, 6 and 9.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-184 of human RAP1B (NP_056461.1).
NCBI Gene ID #:
5908
NCBI Official Name:
Ras-related protein Rap-1b
NCBI Official Symbol:
RAP1B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 21kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Immunology, Signal Transduction
Swissprot #:
P61224
User NOte:
Optimal dilutions for each application to be determined by the researcher.