TRIM25 Antibody

ProSci
Product Code: PSI-14-708
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-14-708-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using TRIM25 antibody (14-708) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
2 / 2
Immunoprecipitation analysis of 200ug extracts of HepG2 cells, using 3 ug TRIM25 antibody (14-708). Western blot was performed from the immunoprecipitate using TRIM25 antibody (14-708) at a dilition of 1:1000.

Western blot analysis of extracts of various cell lines, using TRIM25 antibody (14-708) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunoprecipitation analysis of 200ug extracts of HepG2 cells, using 3 ug TRIM25 antibody (14-708). Western blot was performed from the immunoprecipitate using TRIM25 antibody (14-708) at a dilition of 1:1000.

Further Information

Additional Names:
TRIM25 Antibody: EFP, Z147, RNF147, ZNF147, EFP, Estrogen-responsive finger protein
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IP: 1:50 - 1:100
Background:
The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the cytoplasm. The presence of potential DNA-binding and dimerization-transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 100-400 of human TRIM25 (NP_005073.2).
NCBI Gene ID #:
7706
NCBI Official Name:
tripartite motif-containing 25
NCBI Official Symbol:
TRIM25
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 68kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Growth Factors, Signal Transduction
Swissprot #:
Q14258
User NOte:
Optimal dilutions for each application to be determined by the researcher.