COMT Antibody

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ProSci
Product Code: PSI-14-710
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-14-710-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using COMT antibody (14-710) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
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Immunoprecipitation analysis of 200ug extracts of MCF-7 cells, using 3 ug COMT antibody (14-710). Western blot was performed from the immunoprecipitate using COMT antibody (14-710) at a dilition of 1:1000.
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Western blot analysis of extracts of various cell lines, using COMT antibody (14-710) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
Immunoprecipitation analysis of 200ug extracts of MCF-7 cells, using 3 ug COMT antibody (14-710). Western blot was performed from the immunoprecipitate using COMT antibody (14-710) at a dilition of 1:1000.

Documents

Further Information

Additional Names:
COMT, catechol-O-methyltransferase, HEL-S-98n
Application Note:
WB: 1:500 - 1:2000

IP: 1:50 - 1:100
Background:
Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini. Several transcript variants are formed through the use of alternative translation initiation sites and promoters.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 42-221 of human COMT (NP_009294.1).
NCBI Gene ID #:
1312
NCBI Official Name:
catechol-O-methyltransferase
NCBI Official Symbol:
COMT
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 25kDa, 30kDa
Purification:
Affinity purification
Research Area:
Neuroscience, Signal Transduction
Swissprot #:
P21964
User NOte:
Optimal dilutions for each application to be determined by the researcher.