ECH1 Antibody

ProSci
Product Code: PSI-14-714
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-14-714-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 7
Western blot analysis of extracts of various cell lines, using ECH1 Antibody (14-714) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
2 / 7
Immunohistochemistry of paraffin-embedded human tonsil using ECH1 antibody (14-714) at dilution of 1:150 (40x lens).
3 / 7
Immunohistochemistry of paraffin-embedded human colon using ECH1 antibody (14-714) at dilution of 1:150 (40x lens).
4 / 7
Immunohistochemistry of paraffin-embedded human liver cancer using ECH1 antibody (14-714) at dilution of 1:150 (40x lens).
5 / 7
Immunohistochemistry of paraffin-embedded human gastric cancer using ECH1 antibody (14-714) at dilution of 1:150 (40x lens).
6 / 7
Immunohistochemistry of paraffin-embedded human placenta using ECH1 antibody (14-714) at dilution of 1:150 (40x lens).
7 / 7
Immunohistochemistry of paraffin-embedded human colon carcinoma using ECH1 antibody (14-714) at dilution of 1:150 (40x lens).

Western blot analysis of extracts of various cell lines, using ECH1 Antibody (14-714) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunohistochemistry of paraffin-embedded human tonsil using ECH1 antibody (14-714) at dilution of 1:150 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using ECH1 antibody (14-714) at dilution of 1:150 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using ECH1 antibody (14-714) at dilution of 1:150 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using ECH1 antibody (14-714) at dilution of 1:150 (40x lens).
Immunohistochemistry of paraffin-embedded human placenta using ECH1 antibody (14-714) at dilution of 1:150 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using ECH1 antibody (14-714) at dilution of 1:150 (40x lens).

Further Information

Additional Names:
ECH1, HPXEL
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
This gene encodes a member of the hydratase/isomerase superfamily. The gene product shows high sequence similarity to enoyl-coenzyme A (CoA) hydratases of several species, particularly within a conserved domain characteristic of these proteins. The encoded protein, which contains a C-terminal peroxisomal targeting sequence, localizes to the peroxisome. The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4-dienoyl-CoA isomerase. This enzyme functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-328 of human ECH1 (NP_001389.2).
NCBI Gene ID #:
1891
NCBI Official Name:
enoyl CoA hydratase 1, peroxisomal
NCBI Official Symbol:
ECH1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 36kDa
Purification:
Affinity purification
Research Area:
Signal Transduction
Swissprot #:
Q13011
User NOte:
Optimal dilutions for each application to be determined by the researcher.