HSD17B8 Antibody

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ProSci
Product Code: PSI-14-716
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-14-716-50uL50uL£433.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using HSD17B8 Antibody (14-716) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunofluorescence analysis of U-2 OS cells using HSD17B8 Polyclonal Antibody (14-716) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using HSD17B8 Antibody (14-716) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunofluorescence analysis of U-2 OS cells using HSD17B8 Polyclonal Antibody (14-716) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
D6S2245E, FABG, FABGL, H2-KE6, HKE6, KE6, RING2, SDR30C1, dJ1033B10.9, estradiol 17-beta-dehydrogenase 8, 17-beta-HSD 8, 17-beta-hydroxysteroid dehydrogenase 8, 3-ketoacyl-[acyl-carrier-protein] reductase alpha subunit, 3-oxoacyl-[acyl-carrier-protein] reductase, KAR alpha subunit, estrogen 17-oxidoreductase, ke-6, protein Ke6, really interesting new gene 2 protein, short chain dehydrogenase/reductase family 30C member 1, testosterone 17-beta-dehydrogenase 8
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100
Background:
In mice, the Ke6 protein is a 17-beta-hydroxysteroid dehydrogenase that can regulate the concentration of biologically active estrogens and androgens. It is preferentially an oxidative enzyme and inactivates estradiol, testosterone, and dihydrotestosterone. However, the enzyme has some reductive activity and can synthesize estradiol from estrone. The protein encoded by this gene is similar to Ke6 and is a member of the short-chain dehydrogenase superfamily. An alternatively spliced transcript of this gene has been detected, but the full-length nature of this variant has not been determined.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-261 of human HSD17B8 (NP_055049.1).
NCBI Gene ID #:
7923
NCBI Official Name:
hydroxysteroid 17-beta dehydrogenase 8
NCBI Official Symbol:
HSD17B8
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 27kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Growth Factors, Neuroscience, Signal Transduction
Swissprot #:
Q92506
User NOte:
Optimal dilutions for each application to be determined by the researcher.