PGK2 Antibody

ProSci
Product Code: PSI-14-721
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-14-721-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using PGK2 antibody (14-721) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
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Immunohistochemistry of paraffin-embedded human mammary cancer using PGK2 antibody (14-721) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat testis using PGK2 antibody (14-721) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse testis using PGK2 antibody (14-721) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of C6 cells using PGK2 Polyclonal Antibody (14-721) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of L929 cells using PGK2 Polyclonal Antibody (14-721) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using PGK2 antibody (14-721) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
Immunohistochemistry of paraffin-embedded human mammary cancer using PGK2 antibody (14-721) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat testis using PGK2 antibody (14-721) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using PGK2 antibody (14-721) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using PGK2 Polyclonal Antibody (14-721) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of L929 cells using PGK2 Polyclonal Antibody (14-721) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
PGK2, PGK-2, PGKB, PGKPS, dJ417L20.2, HEL-S-272
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100
Background:
This gene is intronless, arose via retrotransposition of the phosphoglycerate kinase 1 gene, and is expressed specifically in the testis. Initially assumed to be a pseudogene, the encoded protein is actually a functional phosphoglycerate kinase that catalyzes the reversible conversion of 1,3-bisphosphoglycerate to 3-phosphoglycerate, during the Embden-Meyerhof-Parnas pathway of glycolysis, in the later stages of spermatogenesis.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 242-360 of human PGK2 (NP_620061.2).
NCBI Gene ID #:
5232
NCBI Official Name:
phosphoglycerate kinase 2
NCBI Official Symbol:
PGK2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 45kDa
Purification:
Affinity purification
Research Area:
Cancer, Signal Transduction
Swissprot #:
P07205
User NOte:
Optimal dilutions for each application to be determined by the researcher.