HAL Antibody

ProSci
Product Code: PSI-14-749
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-14-749-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using HAL antibody (14-749) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded rat kidney using HAL antibody (14-749) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human lung cancer using HAL antibody (14-749) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human thyroid cancer using HAL antibody (14-749) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse heart using HAL antibody (14-749) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of C6 cells using HAL Polyclonal Antibody (14-749) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using HAL antibody (14-749) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat kidney using HAL antibody (14-749) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using HAL antibody (14-749) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human thyroid cancer using HAL antibody (14-749) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using HAL antibody (14-749) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using HAL Polyclonal Antibody (14-749) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
HAL, HIS, HSTD, histidase
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100
Background:
Histidine ammonia-lyase is a cytosolic enzyme catalyzing the first reaction in histidine catabolism, the nonoxidative deamination of L-histidine to trans-urocanic acid. Histidine ammonia-lyase defects cause histidinemia which is characterized by increased histidine and histamine and decreased urocanic acid in body fluids. Several transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human HAL (NP_002099.1).
NCBI Gene ID #:
3034
NCBI Official Name:
histidine ammonia-lyase
NCBI Official Symbol:
HAL
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 73kDa
Purification:
Affinity purification
Research Area:
Signal Transduction
Swissprot #:
P42357
User NOte:
Optimal dilutions for each application to be determined by the researcher.